Huang, J. Clinical observation on the treatment of acute non-lymphocyte leukemia with co-administration of spreading hedyotis Injectable and chemotherapy. Journal of Henan Traditional Chinese Medicine, 16, Huang, W. Chemical constituents from Hehyotis diffusa. China Journal of Chinese Materia Medica, 33, — China Journal of Chinese Materia Medica, 34, — Ke, Z. The effect of quercetin on the proliferation and apoptosis of human bladder cancer cell BIU The Practical Journal of Cancer, 23, — Li, H.
Studies on the antitumor effect of Hedyotis diffusa on H22 hepatoma in mice. Li, X. Ursolic acid inhibits the angiogenesis of transplanted human lung adenocarcinoma cell line A in nude mice. Journal of Shandong University Health Science , 45, — Li, Y. Treatment of late-stage non-small cell lung cancer with spreading hedyotis extract injection and chemotherapy.
Journal of Henan Traditional Chinese Medicine, 15, Ling, Y. Extraction, separation and assay on polysaccharides from Hedyotis diffusa willd. Biology and Technology, 15, 48— Liu, J. The clinical effect of spreading hedyotis extract injection on primary liver caner. Journal of Medicine Forum, 25, 37— Experimental study on antitumor activities and immunity regulation of Hedyotis diffusa Willd.
Liu, Z. Toxicology in Vitro, 24, — Lu, C. A new acylated flavonol glycoside and antioxidant effects of Hedyotis diffusa. Planta Medica, 66, — Luo, W. Efficacy analysis on the treatment of late-stage cancer by Hedyotis diffusa Injectable with chemotherapy.
Luo, X. Guangzhou: Guangdong Science and Technology. Meng, Q. F, Ren, F. Effect of polysaccharides from Hedyotis diffusa willd. On cell line Bel In the present study, we attempted to investigate the apoptotic activity of crude extracts of the herb as well as the possible molecular pathways. Methods: We incubated human promyelocytic leukemia cell line HL60 cells with ethanol or aqueous extracts of the herb, and determined the levels of intracellular superoxide at 2 and 4 hours as well as caspase activity at 3, 6 and 8 hours using photospectrometry.
Revised 09 Feb Accepted 11 Feb Published 10 Mar Abstract Oldenlandia diffusa OD is commonly used with various diseases such as cancer, arthritis, and autoimmune disease. Introduction Hepatocellular carcinoma HCC is the fifth most common cancer and the second leading cause of cancer death worldwide [ 1 , 2 ].
Materials and Methods 2. Cell Viability Assay Cell viability was measured using a Cell Counting Kit-8 CCK-8; Dojindo Laboratories allowing sensitive colorimetric assay by using highly water-soluble tetrazolium salt that is reduced by dehydrogenases in living cells to give a colored product formazan.
Animal Model The experimental protocols used in this study were designed according to the animal experimental guidelines established by the Institutional Animal Care and Use Committee of Catholic University Medical School.
Histological Examination 28 days after OD treatment, animals from each group were sacrificed for histological examination. Result 3. Figure 1. The cytotoxic effect and antiproliferative activity were measured by CCK-8 cytotoxicity assay and immunoreactive cells such as Ki67 and caspase OD treatment in huh7 and hepG2 cells reduced kipositive cells and increased caspase-3 immunoreactivity.
Columns, mean; bar, SE. Figure 2. Effect of OD on cell migration by scratch wound migration assay a , b , and c. Figure 3. We recorded starting from day 0 after the last DEN injection. We record death rate between 20 days and 36 days c. Columns, mean; bar, SD. Figure 4. Comparison of tumor number was categorized by tumor size 28 days after OD treatment b.
Also, a larger tumor burden in OD treated group was observed less than control group b. Figure 5. Inhibition of tumor cell proliferation by OD. Quantification of PCNA-positive cells in the tumor area b. Figure 6. The large tumor observed in two sites a. Except for tumor site, the rest of the liver tissue was similar to the normal liver morphology. Also, no cirrhosis was shown and normal liver cells exist j , n.
Supplementary Materials. References M. Taha, A. Abdul, R. Abdullah, T. Ibrahim, S. Abdelwahab, and S. Jemal, F. Bray, M. Center, J. Ferlay, E. Ward, and D. Thorgeirsson and J. Kern, K. Breuhahn, and P. Moudgil, D. Redhu, S. Dhanda, and J.
Branda and J. Hanajiri, H. Mitsui, T. Maruyama, N. Hashimoto, M. Sata, and M. Park, H. Park, S. Kang et al. Xu, X. Chen, Q. Use of this Web site does not create an expressed or implied physician-patient relationship.
Memorial Sloan Kettering does not record specific website user information and does not contact users of this website. You are hereby advised to consult with a physician or other professional health-care provider prior to making any decisions, or undertaking any actions or not undertaking any actions related to any health care problem or issue you might have at any time, now or in the future. In using this website you agree that neither Memorial Sloan Kettering nor any other party is or will be liable or otherwise responsible for any decision made or any action taken or any action not taken due to your use of any information presented at this website.
This will help them manage your care and keep you safe. Oldenlandia diffusa has shown anticancer effects in lab studies, but human data are lacking. It is used in traditional Chinese medicine TCM for the treatment of liver diseases, snake bites, and tumors. Laboratory studies suggest that this herb may inhibit the growth of cancer cells and stimulate the immune system to destroy or engulf tumor cells.
Animal studies show that certain chemicals found in this herb may help lower cholesterol and have anti-inflammatory effects. More research is necessary to confirm whether this herb can be used safely and effectively for any condition in humans. Poor quality control has been reported with products containing of O. It is used in combination with other herbs for the treatment of hepatitis, snake bites 1 , and tumors of the liver, lung, stomach 1 , and rectum 2.
Studies conducted in vitro and in animals suggest that O. Oleanolic and ursolic acids, compounds isolated from O. Ursolic acid also showed anti-inflammatory effects in mice with rheumatoid arthritis 9. Human data are lacking.
Studies conducted in vitro and in animals have shown that O. Immunomodulating effects may occur through immune system stimulation to kill or engulf tumor cells 5. Quality control issues have been reported.
0コメント